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Abstract
Mumps virus is a single-stranded negative sense RNA virus, and a member of the Paramyxoviridae family. This usually self-limited disease is endemic worldwide and occurs primarily in children and adolescents. Twenty-five to 30% of infections are asymptomatic. The most frequent clinical presentation is nonsuppurative parotitis, which occurs in up to 60-70% of persons infected. Meningitis, oophoritis and epididymo-orchitis represent the most common complications. Symptoms and complications might vary between immunized and unimmunized patients. Diagnosis relies on serology, viral culture, or molecular testing, such as real-time reverse-transcription polymerase chain reaction (rRT-PCR). Since the beginning of 2023, a mumps outbreak has hit Zimbabwe's major cities, Harare and Bulawayo. This viral infection is mostly affecting children in schools (Magurano et al. 2018; Savage et al. 2004). Between January and August 2023, specimens from suspect cases were submitted to National Microbiology reference Laboratory for mumps serology, culture and/or real-time reverse transcriptase PCR (rRT-PCR) testing. These samples will be requested for rRT-PCR and genotyping at the National Genomics Centre. Whole genome sequencing will be performed on the outbreak culture isolates. This research project will bring to light the mumps virus strains causing an outbreak in Zimbabwe. Futhermore it will bring out the importance of molecular techniques in the diagnostics of pathogens to inform Public health on pandemics.
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https://doi.org/10.28919/jmcs/7943
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https://doi.org/10.9734/ijtdh/2022/v43i1030616
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